Figure 5.

HSF response to genotoxic effect of K₂CrO₄. Cells were seeded onto a 24-well plate and cultured for 48 h. Then 4 or 6 µM of K₂CrO₄ was added, cells were incubated for 24 h (point 24 h), the medium was replaced, and incubation was continued for up to 72 h (point 96 h). Cells of three to six wells were examined for each K₂CrO₄ concentration and each control variant. (A) Change for the cell DNA on the plate. (B) Change for the cell-free DNA in the medium. (C) Change in caspase 3 activity in the protein lysate of the cells. (D) Change in the f-SatIII content in the DNA. *p < 0.05.