Figure 2.

Inhibition of CD147 attenuates post-stroke lung vascular permeability and edema through inhibition of MMP-9 activity. (A) Representative pictures and quantitative analysis of Evans blue leakage in the lung tissue at 24 h after stroke. L: left lung (one lobe), R: right lung (four lobe). Bar = 5 mm. n = 6 per group. (B) Lung water content at 24 h after stroke. n = 6 per group. (C) Representative images of western blots showing MMP-9 protein levels in the lung tissues and semi-quantitative analysis of immunoblots. n = 4 per group. (D) Double immunostaining for MMP-9 and neutrophils in the lung sections in indicated groups at 24 h after stroke (left panel). Neg.Ctrl indicates negative control. Bar = 100 μm. The number of the cells double positively stained for neutrophils and MMP-9 per mm² was counted (right panel). n = 6–7 per group. ^*P < 0.05.