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. 2019 Aug 7;10:853. doi: 10.3389/fneur.2019.00853

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Copyright © 2019 Jin, Liu, Wang, Zhong and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of CD147 modulates the changes of leukocyte subpopulations in the lung after stroke. (A) Representative flow cytometric dot plots of R1: total leukocytes (CD45⁺), R2: neutrophils (CD45⁺CD11b⁺Ly6G⁺), and R3: ly6C^Hi monocytes (CD45⁺CD11b⁺Ly6G⁻Ly6C^Hi) in the isolated lung cells from indicated groups. Lung infiltrating cell was isolated using enzyme digestion method at 24 h after stroke. (B) Representative flow cytometric dot plots of lung conventional dendritic cell (cDC, identified as CD11c^Hi/IA/IE^Hi) and macrophage (MΦ, CD11c^Hi/IA/IE^Lo) populations. (C) Representative flow cytometric dot plots of B cell population (B220⁺). (D,E) The number and frequency of cell subpopulations in the indicated groups. n = 6 per group. ^*P < 0.05.