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Y78 phosphorylation status regulates the nuclear export of NEP-M1-vRNP complex. The co-localizations of NP with M1/NEP/PB1 were examined by IFAs. The 12-plasmid reverse genetic system containing WT, Y78F, or Y78E NP plasmid was transfected into 293 T cells. At 12 and 36 h.p.t., cells were fixed and stained with anti-NP (red) and anti-M1/NEP/PB1 antibodies (green). The nucleus was stained with DAPI (blue).
