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. 2019 Aug 7;10:1814. doi: 10.3389/fmicb.2019.01814

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Copyright © 2019 Liu, Wang, Zhang and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The influence of MafB inhibition to CD14⁺ monocytes from chronic hepatitis C patients (n = 10) on HCVcc infected Huh7.5 cells. CD14⁺ monocytes were transfected with control siRNA or MafB siRNA and were co-cultured with HCVcc infected Huh7.5 cells in direct and indirect contact manners for 48 h. Supernatants were harvested for further analysis. (A) HCV RNA was quantified by real-time PCR and was compared among groups. Significances were determined using SNK-q test. (B) Percentage of target cell death was determined by measuring LDH expression and was compared among groups. Significances were determined using one-way ANOVA. (C) IFN-α1 and (D) IFN-β were measured by ELISA and were compared among groups. Significances were determined using SNK-q test and Dunn’s multiple comparison test, respectively. Individual level for each enrolled subject was shown.