Figure 5.

The influence of MafB inhibition to CD14⁺ monocytes from chronic hepatitis C patients (n = 9) on CD4⁺ T cells differentiation. CD14⁺ monocytes were transfected with control siRNA or MafB siRNA and were co-cultured with autologous CD4⁺ T cells in direct and indirect contact manners for 48 h. Cells were harvested for flow cytometry analysis. (A) Representative flow dots for CD4⁺IFN-γ⁺ (Th1 phenotype), CD4⁺IL-4⁺ (Th2 phenotype), and CD4⁺IL-17⁺ (Th17 phenotype) in MafB siRNA transfected CD14⁺ monocytes co-cultured with CD4⁺ T cells. Comparison of (B) CD4⁺IFN-γ⁺, (C) CD4⁺IL-4⁺, and (D) CD4⁺IL-17⁺ percentage among groups. Significances were determined using one-way ANOVA and SNK-q test. Individual level for each enrolled subject was shown.