Figure 6.

Type I IFN production by MafB expression plasmid transfection in CD14⁺ monocytes from chronic hepatitis C patients (n = 6). CD14⁺ monocytes were electroporated by blank plasmid or MafB expression plasmid. Cells and supernatants were harvested 48 h post stimulation. (A) MafB protein expression was measured by Western blot in untransfected, blank plasmid transfected, and MafB expression plasmid transfected CD14⁺ monocytes. Comparison of (B) IFN-α1 and (C) IFN-β expression in untransfected, blank plasmid transfected, and MafB expression plasmid transfected CD14⁺ monocytes. Significances were determined using one-way ANOVA and SNK-q test. Individual level for each enrolled subject was shown.