REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Top10	Fischer scientific (UK)	C404010
BL21(DE3)	Sigma-Aldrich, Merck	CMC0014
Chemicals, Peptides, and Recombinant Proteins
IBMX	Fischer scientific (UK)	PHZ1124
LY-83,583	Enzo Life Sciences, CAS: 91300-60-6	ALX-550-002-M005
Glutathione Sepharose 4B	(GE Healthcare, Amersham, UK)	GE17-0756-01
Brilliant III Ultra-Fast SYBR qPCR mastermix	Agilent	600882
Pectolyase Y23	Fischer scientific (UK)	ICN320951
Cellulase RS	SERVA Electrophoresis GmbH, Germany	16420
Cellulysin	Calbiochem, Merck, Feltham, UK	219466
BSA	Sigma-Aldrich, Merck	A2153
MES sodium salt	Fischer scientific (UK)	71119-23-8
IPTG	Fischer scientific (UK)	50-121-7428
Critical Commercial Assays
ELISA cGMP immunodetection	Enzo Life Sciences LTD., Exeter, UK	ADI-900-013
Cyclic Nucleotide Phosphodiesterase assay kit	Enzo Life Sciences LTD., Exeter, UK	BML-AK800-0001
Experimental Models: Organisms/Strains
Arabidopsis seeds pde1-1	http://arabidopsis.info/	SALK_009905
Arabidopsis seeds pde1-2	http://arabidopsis.info/	SALK_104566
Arabidopsis seeds Columbia-0	Alistair Hetherington lab	N/A
Physcomitrella patens	Jill Harrison’s lab [26]	N/A
uvr8-6	Favory et al. (2009) [25]	N/A
Oligonucleotides
Pde1f	This paper	TGGAAATTGTGGAGCTTGCAG
Pde1r	This paper	TCGTTAGTATCTTCGTCTTCTTGG
act2qrtf	This paper	TTCCAGCAGATGTGGATCTC
act2qrtr	This paper	TCTGTGAACGATTCCTGGAC
pde1GstBamHI_F	This paper	AAAGGATCCATGGCGGCGAAGACGATGA
pde1GstXhoI_R	This paper	AAACTCGAGTCAAGTTGACCCATCCCACT
EF1alpha_F	This paper	TGTGCTGTTCTTATCATTGACTCC
EF1alpha_R	This paper	TGGCATCCATCTTGTTACAACAG
Recombinant DNA
GST::CN-PDE1	EF1alpha_R	This paper
pGEX6P-1 vector	(GE Healthcare, Amersham, UK)	27-1542-01
Software and Algorithms
SIMPLE PCI 6.1.2	https://www.hamamatsu.com/	N/A
MXPro 3005 real time PCR system	Agilent	N/A
Prism 6	GraphPad Software	N/A
HMMER3 suite	[27]	N/A
Other
LEDs 385nm	Ushio Epitex inc., Kyoto, Japan	L385R
LEDs 470nm	Farnell	2414443
LEDs 660nm	Farnell	1142564
LEDs 735nm	Ushio Epitex inc., Kyoto, Japan	L735