FIG 5.

PA14-1 RSCVs have increased biofilm formation and fitness relative to the ancestor PA14-1. (A) PA14-1 RSCVs were grown in 96-well plates for 24 h. Biofilm biomass was stained and quantified by crystal violet. Biofilm biomass levels were expressed as a percentage relative to the ancestor strain, which was set at 100% for each replicate. Data are presented as mean plus SD (n = 3). Significance was determined using a one-way ANOVA: *, P value of <0.05; **, P value of <0.01; ***, P value of <0.001. (B) Fitness of representative PA14-1 RSCVs relative to the ancestor PA14-1. PA14ΔwspF and its isogenic parent PA14, compared to PA14-1, were used as controls. Strains were grown for 48 h as either a planktonic culture or biofilm, and selection of the RSCVs relative to PA14-1attB::lacZ was determined by calculating the selection rate according to equation 1. Data are presented as mean ± SD (n = 5).