TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or characteristic(s) | Reference or source |
|---|---|---|
|
P. aeruginosa strains used in the infectiona |
||
| PA14-1 | Barcode CAAAAGGACA; Gent | |
| PAO1-B11 | Barcode GTGTCGTGGG; Gent | |
| B23-2 | Wound isolate; barcode GCCTATTGTG; Gent | Lubbock, TX |
| CF18-1 | Nonmucoid CF isolate; barcode GTTACGTCAA; Gent | 60 |
| MSH10-2 | Water isolate; barcode TATCAGATTT; Gent | 60 |
| S54485-1 | UTIb isolate; barcode TTAAACTAGG; Gent | 60 |
| P. aeruginosa strains | ||
| PA14 | ||
| PA14ΔwspF | Clean wspF deletion | |
| PAO1 | ||
| PAO1ΔwspF | Clean wspF deletion (JJH356) | |
| PA14-1attB::lacZ | lacZ from miniCTX-lacZ introduced at the attB site | This study |
| RSCV-2 wt-wspA | Variant wspA replaced by the wild-type allele | This study |
| RSCV-1 wt-wspA | Variant wspA replaced by the wild-type allele | This study |
| RSCV-40 wt-wspA | Variant wspA replaced by the wild-type allele | This study |
| E. coli strains | ||
| NEB5-α | NEBc | |
| S17 | NEB | |
| Plasmids | ||
| pEX18Ap | Gene deletion vector | 47 |
| pEX18Ap::wt-wspA | wspA-complementing construct | This study |
| pCdrA::gfp | CdrA promoter fused to gfp; Carb | 31 |
| pMH487 | Empty vector for pCdrA::gfp; Carb | 31 |
| pJN2133 | PA2133 cloned into pJN105; Gent | 32 |
| pHERD20T | Empty vector; Carb | 56 |
| pHERD2133 | PA2133 from pJN2133 cloned into pHEDR20T; Carb | This study |
| miniCTX-lacZ | Tet | 58 |
a
All strains used in the infection are resistant to gentamicin. Barcodes are located at the Tn7 site on the genome.
b
UTI, urinary tract infection.
c
NEB, New England Biolabs.