TABLE 3.
Mutations identified in PA14-1 RSCVs
| Sample |
Driver mutation |
Secondary mutation |
|||||
|---|---|---|---|---|---|---|---|
| Day | RSCV no. | Wound no. | Gene | Mutationa | Frequency (%) | Gene | Mutation |
| 3 | 1 | 1 | wspA | 285-298del | pslO-pslE | Δ14,299 bp | |
| 2 | 2 | wspA | 285-298del | ||||
| pslO-pslE | Δ14,299 bp | ||||||
| 4 | 1 | wspA | 285-298del | PA14_13130/PA14_13140 | TNN→TGC | ||
| fabI/ppiD | TTC→TCC | ||||||
| Day 3 summary | wspA | 285-298del | 100 | ||||
| 14 | 3 | 4 | wspA | 285-298del | |||
| 6 | 4 | wspA | 285-298del | ||||
| 7 | 4 | wspA | 285-298del | ||||
| 8 | 4 | wspA | 285-298del | ||||
| 9 | 4 | wspA | 285-298del | ||||
| 10 | 4 | wspA | 285-298del | ||||
| 12 | 4 | wspA | 285-298del | CRISPR–Cas1/hpb | +60bp | ||
| 13 | 1 | wspA | 285-298del | Glutamyl-tRNA reductase | L71L (CTG→TTG) | ||
| 14 | 4 | wspA | 285-298del | fabI/ppiD | TTC→TCC | ||
| 16 | 4 | wspA | 285-298del | ||||
| 17 | 4 | wspA | 285-298del | ||||
| 20 | 4 | wspF | V154fs | ||||
| 24 | 1 | wspA | 285-298del | ||||
| 27 | 1 | wspA | 285-298del | ||||
| 28 | 2 | wspA | 285-298del | ||||
| 36 | 3 | wspF | V154fs | ||||
| 37 | 3 | wspA | 285-298del | ||||
| 38 | 3 | wspF | V154fs | ||||
| 86 | 3 | wspA | 285-298del | CRISPR–Cas1/hp | +60bp | ||
| Day 14 summary | wspA | 285-298del | 84.2 | ||||
| wspF | V154fs | 15.8 | |||||
| 28 | G→TT 585bp | ||||||
| wspD | CT→AG 588-589bp | ||||||
| 40 | 1 | wspA | 285-298del | S197S (TCG→TCA) | |||
| PA14_54090 | A248V (GCG→GTG) | ||||||
| 42 | 3 | wspA | 285-298del | ||||
| 43 | 3 | wspA | 285-298del | ||||
| 45 | 3 | wspA | 285-298del | ||||
| 87 | 2 | wspA | 285-298del | ||||
| Day 28 summary | wspA | 285-298del | 100 | ||||
a
Deleted amino acid residues are indicated. del, deletion; fs, frameshift.
b
hp, hypothetical protein.