FIG 2.

Mutations in an oligopeptide permease operon lead to early dispersal. (a) Cartoon depicting an evolution experiment to select for an M. smegmatis strain that disperses earlier than WT. (b) Aggregation curve of WT M. smegmatis, the passage 60 isolate, and the Δopp mutant grown in rich medium plus 0.2% glucose. The top panel shows the aggregated fraction, and the bottom panel shows the planktonic fraction. Data are representative of n = 3 trials. (c) Aggregation curve of WT M. smegmatis and the Δopp mutant grown in glycerol defined medium. The top panel shows the aggregated fraction, and the bottom panel shows the planktonic fraction. Data are representative of n = 2 trials.