FIG 3.

Carbon depletion leads to dispersal. (a) Aggregation curve of WT M. smegmatis in rich medium with no glucose, 0.2% glucose, or 0.6% glucose. The top panel shows the aggregated fraction, and the bottom panel shows the planktonic fraction. Data are representative of n = 3 trials. (b) Aggregates harvested from 48-h-old rich medium cultures (Time 0) were resuspended in conditioned medium (filter sterilized from 52-h-old rich medium cultures), PBS, or conditioned medium plus 0.6% glucose and grown for 12 h. Each bar is an average from biological triplicates, and error bars represent standard deviations. Asterisks represents P < 0.05 by the Student t test.