TABLE 1.

Nontransposon ORFs mutated in passage 40 and passage 60 isolates relative to the passage 0 isolate

Passage no. and gened	Function	Mutation type	Mutation
Passage 40
MSMEG_1808	SufE	Missense	Glu39Val
MSMEG_2148	HNHe endonuclease domain-containing protein	Frameshift	Ser534fsa
MSMEG_5061	Extracellular solute binding protein	Missense	Ser249Pro
MSMEG_5808	Binding protein-dependent transporter	Missense	Arg117Cys
MSMEG_6397	Hypothetical protein	Missense	Ser21Pro
MSMEG_6430	Hypothetical protein	Missense	Thr371Lys
MSMEG_6821	NLP/P60 family protein	Missense	Gln2017Arg
Passage 60
MSMEG_0639	Oligopeptide transport ATP-binding protein OppF	Frameshift	Lys12fsb
MSMEG_0640	Oligopeptide transport ATP-binding protein OppD	Missense	Phe96Leu
MSMEG_2148	HNH endonuclease domain-containing protein	Missense	Pro380Arg
MSMEG_2148	HNH endonuclease domain-containing protein	Frameshift	Ser534fsa
MSMEG_3677	Serine/threonine protein kinase	Silent	Val320Val
MSMEG_4217	DivIVA protein	Missense	Glu107Gly
MSMEG_5061	Extracellular solute binding protein	Frameshift	Glu225fsc
MSMEG_5395	Sensor histidine kinase KdpD	Missense	Arg627Cys
MSMEG_6497	Hypothetical protein	Missense	His43Gln

^aMSMEG_2148 is 544 amino acids. Ser534 frameshift hypothetically replaces the 8 C-terminal amino acids with a different 23-amino-acid sequence.

^bMSMEG_0639 is 336 amino acids. Lys12 frameshift hypothetically replaces the 325 C-terminal amino acids with a different 24-amino-acid sequence.

^cMSMEG_5061 is 465 amino acids. Glu225 frameshift hypothetically replaces the 241 C-terminal acids with a different 236-amino acid-sequence.

^dBold text indicates the genes that were mutated in WT to test for aggregation defects.

^eHis-Asn-His (HNH).