FIG 4.

Antibody responses after AuNP-protein immunization. (A) LomW-specific total IgG titers and (C) secretory IgA titers, as well as (B) EscC-specific total IgG antibody responses and (D) secretory IgA titers, were assessed via ELISA, with endpoint titers defined as twice the standard deviation (SD) of the levels measured for naive sera. Sera and fecal samples were collected 2 weeks after the last immunization. Samples taken from the mice immunized with the combination formulation were used to assay anti-LomW and anti-EscC antibody titers. All antibody data are expressed as mean ± SEM of results from n = 8 mice per group. Significant differences in IgG and IgA titers were determined via one-way ANOVA followed by Tukey’s post hoc test (*, P < 0.05; **, P < 0.001; ***, P < 0.0001).