Table 1.
Primers used in the study
| Primer | Sequence | Restriction site (underlined) | Notes | ||
|---|---|---|---|---|---|
| Pdc1 | gtccagattggatccaagcatcacttcgcag | BamHI | |||
| Pdc2 | cttcagtgcaagcttcacgaccgatgaagc | HindIII | |||
| TetAge1 | cagcttatcaccggtaagctttaatgcgg | AgeI | |||
| TetAge2 | gattcattctgctaaccggtaaggcaaccc | AgeI | |||
| Gap1 | gacaatgagctcggaacggtatactg | SacI | |||
| Gap2 | caactttaaccgccatggatcctctc | BamHI | |||
| SglucPdc_fwd | agaggatccATGACCACTGGTCGTATGTCTCGTCGAGAATGCCTTTCCGCAGCTGCCATAGTGCCTATTGCCGCTATGACAGCCACCGCCACTATTACAGGATCAGCTCAGGCTatgagttatactgtcggtac | BamHI | 105 bp fragment encoding the 35 aa signal sequence of gluconolactonase shown in capital letters | ||
| Pdc_rev | gtttattaagcttctagaggagc | HindIII |