Figure 4.

Analysis of the influence of an increasing concentration of phosphatidylserine (a), phosphatidic acid (b), phosphatidylglycerol (c) and phosphatidylcholine (d) on the enzymatic activity of Amuc_1686 (●) and Amuc_1686_short (○). Control experiments with methanol with an equimolar final concentration had no negative influence on the enzymatic activity. The reaction assays contained 1 µg Amuc_1686, 5 mM para‐nitrophenyl‐β‐d‐galactopyranoside and were performed in a combined buffer (50 mM) at pH 7.5 and at a temperature of 37°C. The activity was measured photometrically at 420 nm. The experiments were conducted at least in triplicates using three different Amuc_1686 preparations. One representative experiment is shown. The average specific activity of Amuc_1686 was 20.2 ± 2.5 U mg^-1 protein (100%)