Table 1.
Round | Input RNA (pmol) | C. albicans (cells) | Washing |
---|---|---|---|
1 | 100 | 5 × 109 | 100 μl 5 times, 1 min each |
2 | 25 | 5 × 108 | 100 μl 5 times, 3 min each |
3 | 25 | 5 × 108 | 100 μl 5 times, 3 min each |
4 | 12.5 | 5 × 107 | 100 μl 5 times, 5 min each |
5 | 12.5 | 5 × 107 | 100 μl 5 times, 5 min each |
6 | 6.25 | 5 × 106 | 100 μl 5 times, 10 min each |
7 | 6.25 | 5 × 106 | 100 μl 5 times, 10 min each |
8 | 3.13 | 5 × 105 | 100 μl 5 times, 15 min each |
9 | 3.13 | 5 × 105 | 100 μl 5 times, 15 min each |
10 | 1.56 | 5 × 104 | 100 μl 5 times, 15 min each |
11 | 1.56 | 5 × 104 | 100 μl 5 times, 20 min each |
Saccharomyces cerevisiae was used in a subtraction step at rounds 3, 5, and 10.