Figure 7.

Blockade of TRAF3 mitigated H/R‐caused injury in cells. IEC‐6 cells were transfected with TRAF3 siRNA in antibiotic‐free cell medium for 24 hr. (a) The viability and morphology of IEC‐6 cells after transfected with TRAF3 siRNA. (b) The expression level of TRAF3 in cells after transfected with TRAF3 siRNA. (c) Cell apoptosis after transfection of TRAF3 siRNA in IEC‐6 cells. (d) The mRNA level of TRAF3 after transfected with TRAF3 siRNA in IEC‐6 cells. (e) The protein level of TRAF3 after transfection of TRAF3 siRNA in IEC‐6 cells by immunofluorescence staining (200× magnification). (f) The protein levels of TRAF3, TAK1, p‐TAK1, NF‐κB (p65), BAX, and Bcl‐2 after transfection of TRAF3 siRNA in IEC‐6 cells by western blotting assay. (g) The mRNA levels of IL‐1β, IL‐6, and TNF‐α after transfection of TRAF3 siRNA in IEC‐6 cells. All data are expressed as the mean ± SD (n = 5). *P < .05, significantly different from control group; ^# P < .05, significantly different from H/R group; n.s., not significant