Figure 3.

UNC1999 prevented PDGF‐BB‐induced VSMC proliferation. (a) VSMCs were treated with PDGF‐BB (40 ng·ml⁻¹) with or without UNC1999 (0.5, 1, and 3 μM, respectively) for 36 hr. Western blot analysis of protein levels of H3K27me3, total H3, and GAPDH. (b–g) VSMCs were treated with PDGF‐BB (40 ng·ml⁻¹) with or without UNC1999 (3 μM) for 36 hr. (b) MTS analysis of cell viability. (c) Representative immunofluorescence staining of Ki67 and DAPI (left panel, scale bar = 50 μm); quantification of percentage of Ki67‐positive cells (right panel). (d,e) Cell cycle determination by flow cytometry. Levels of mRNA (f) and protein (g) of PCNA. Data are mean ± SEM, n = 5, *P < .05. Ctrl, control