Figure 1.
Levels of NO released by MN‐08 in vitro and in vivo. (a) Formation of total NO by MN‐08 (40 μM) and ISDN (40 μM) incubated in PBS buffer with 25‐mM L‐cysteine. (b) NO levels released in rat plasma. After MN‐08 (25 mg·kg−1) or ISDN (3 mg·kg−1) was given intravenously through the tail vein, blood samples were drawn from the jugular vein at 0, 0.25, 0.5, 1, 2, and 4 hr after drug administration. The NO released from MN‐08 or ISDN was assayed with Griess reagent. The absorbance was measured at 540 nm by microplate reader. Data shown are means ± SD (n = 6)