Figure 3.

MN‐08 increased RBCs velocity in the cerebral cortex of mice. (a) Diagram of the experimental procedure. Mice were injected with saline (0.02 ml·min⁻¹) for 5 min and then treated with MN‐08 (12 mg·kg⁻¹) for 10 min via tail injection. (b) Fluorescence images of isothiocyanate dextran labelled vessels in the mouse cerebral cortex with a 40× objective (left image) and a magnified image (right). The RBCs velocity was measured using line scan model of two‐photon microscopy. (c) Diagram showing the calculation of RBCs velocity. (d) The RBCs velocity before and after MN‐08 treatment. Data shown are means ± SEM (n = 6). *P < .05, significantly different from saline; paired, two‐tailed Student's t test