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. 2019 Jun 24;10(17):3926–3932. doi: 10.7150/jca.31345

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GPC6 increased the capacity of nasopharyngeal carcinoma (NPC) cells to form colonies and become invasive in vitro. 5-8F and HNE2 cells were transfected with pIRESneo3-GPC6 or an empty vector (negative control, NC), and allowed to proliferate for 24 h before being subjected to (A) a colony formation and (B) a Matrigel invasion assay. The results of these analyses showed that GPC6 overexpression increased both the number of colonies that were formed by the cells, and the invasive capacity of the cells, respectively.