Figure 1.

Construction and analysis of the A549RR, NRP1^LowA549 cell models and 3D-culture model. Changes in NRP1 expression during construction of the A549RR model at the mRNA (A) and protein level (B). Effect of 10 Gy X-ray irradiation on survival of A549RR cells (C) and colony forming ability (D). NRP1 expression in the A549RR and NRP1^LowA549 cell models at the mRNA (E) and protein level (F). Changes in the morphological and cytoskeleton of A549 cells grown in Matrigel matrix were clearly distinguished by bright field conditions or phalloidin and DAPI staining: F-actin in red and DAPI-stained nuclei in blue (G). Changes in the EMT-related marker proteins vimentin, N-cadherin and E-cadherin in 2D and 3D culture (H). *p<0.05, ***p<0.001 vs control group; ^{^^^}p<0.001 vs A549 IR group (n=3).