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. 2019 Jul 8;10(17):4017–4030. doi: 10.7150/jca.28163

Figure 7.

Figure 7

Effect of NRP1 on TGF-β/Smad signaling pathway in the tumor migratory microenvironment. The effect of NRP1 on the secretion of TGF-β in the migratory microenvironment. 2D co-culture systems (A), In tumor tissue (D); mRNA expression of TGF-β and smad7 in 2D co-culture systems (B-C). qRT-PCR was performed to measure the regulatory effect of NRP1 on mRNA expression of TGF-β/Smad signaling pathway in tumor tissue. TGF-β (E), Smad7 (F), Smad3 (G), Smad2 (H), NRP1 (I); Western blot was applied to investigate the regulation of NRP1 on TGF-β/Smad signaling pathway in tumor tissues (J); *p<0.05 vs control; & p<0.05, && p<0.001 vs NRP1LowA549 co-culture group; ^ p<0.05, ^^^ p<0.001 vs A549 con group (n = 3).