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. 2019 Jul 10;10(17):4142–4150. doi: 10.7150/jca.30493

Figure 4.

Figure 4

DPP7 knockdown induces cell apoptosis in HepG2 cells (A) RT-PCR analysis of cell cycle regulated genes in HepG2 and HepG2.2.15 cells. GAPDH was used as a reference gene. Data shown represents the mean ± SEM values from three independent experiments (*p < 0.05, ***p < 0.001). (B) Western blot analysis of cell cycle regulated genes. Quantification results were shown in the right. GAPDH was used as a reference gene. Data shown represents the mean ± SEM values from three independent experiments (**p<0.01, ***p < 0.001). (C) Verification of DPP7 siRNA knockdown efficiency by RT-PCR and western blot. Quantification results were shown in the upper. Data shown represents the mean ± SEM values from three independent experiments (***p < 0.001). (D) Knockdown of DPP7 induces increased cell apoptosis in HepG2 cells. Data shown represents the mean ± SEM values from three independent experiments (***p < 0.001). (E) Knockdown of DPP7 in HepG2 cells leads to increased Bax/Bcl2 ratio as measured by western blot. Quantification results were shown in the right. GAPDH served as a loading control. Data shown represents the mean ± SEM values from three independent experiments (***p < 0.001).