Figure 3.

Characterization of protease. (A) Temperature stability of protease. Enzyme was preincubated at various temperatures followed by modified Lowry assay at 37°C. (B) Enzyme activity at 37°C after preincubation of protease at 60°C to determine stability of protease at 60°C. (C) Effect of pH on protease activity. Assay was performed at different pH values at 37°C. (D) Effect of temperature on enzyme activity. Assay was performed at pH 12 with varying temperatures. (E) Effect of chloride metal salts (10 mM) on enzyme activity at pH 12, 37°C. (F) Effect of solvents (10% v/v) on enzyme activity at pH 12, 37°C. (G) Protease activity on different substrates. Columns and error bars above column represent mean ± standard error of the means, respectively.