Table 1.
Purification of protease from Microbacterium sp. SKS10.
| Purification step | Total activity (U)a | Total protein (mg)b | Specific activity (U/mg) | Yield (%) | Fold purification |
|---|---|---|---|---|---|
| Crude enzyme | 2004.8 | 65.2 | 30.748 | 100 | 1 |
| Ammonium sulfate precipitation | 1317.5 | 37.809 | 34.847 | 65.7 | 1.13 |
| Gel permeation chromatography | 35.568 | 0.222 | 160.21 | 1.77 | 5.21 |
a
Enzyme activities were determined by using casein as a substrate spectrophotometrically at 660 nm.
b
The protein concentration was estimated using Bradford's method.