Figure 2.

Role of intracellular Ca²⁺ mobilization in lactate-induced GPR81 signaling in pDCs. (A) Primary pDCs were stained with calcium binding dye- Fluo-3AM and in the presence (thick line) or absence (thin line) of 1 mM EGTA acquired on a flow cytometer, before and after addition of K⁺-lactate. The arrow represents the addition of 10 mM K⁺-lactate and the Y-axis represents the fluorescence emitted by the dye. The figures are representative of 3 independent experiments. (B,C) K⁺-lactate was added to pDC cultures preincubated with indicated concentrations of EGTA (B), CamKII-I (C), and CsA (D), and then stimulated with CpGA. After 20 h, IFNα ELISA was done with the culture supernatants. n = 4–6 from 2 to 3 independent experiments and two-tailed paired Student's t-test (^*p < 0.05, ^**p < 0.005 and ns, not significant).