Fig. 4.
Relative activation and dose responses of Olfr90 ligands. A: ligands found to activate Olfr90 are listed in order of the degree of activation relative to 2-methyl-4-propyl-1,3-oxathiane (MPOT). Odorant molecules were all tested at 0.5 mM; compound structures are shown for reference. Those listed in bold demonstrated activation above 80%. B−E: dose-response curves for Olfr90 (◇; tagged with Rho and Lucy) are shown for MPOT (B), 1-octen-3-ol (C), 3-octanol (D), and 2-octanol (E) when coexpressed with RTP1S, receptor-transporting protein 1S (RTP1S). Olfr90 demonstrated the highest affinity for 2-octanol with an EC50 value of ~75 μM. AU, arbitrary units. *P < 0.02 vs. no compound using one-way ANOVA.