Fig. 5.

Gene expression analyses in the bladders. A: quantitative PCR results for oxidative stress (left) and proinflammatory (right) markers. B: quantitative PCR results for genes known to be involved in detrusor smooth muscle (DSM) contractile and relaxation function. Values are means ± SE of fold differences (with the control group taken as 1). Results were normalized with β-actin (Actb) and TATA box-binding protein (Tbp; N = 4–5 per group). Open and closed bars represent control and doxorubicin (DOX)-treated groups, respectively. *P < 0.05 vs. the control group. C and D: Western blot analysis of genes related to DSM contractility (C) and acto-myosin filaments (D). Nox1 and Nox2, NADPH oxidase 1 and 2; Duox1 and Duox2, dual oxidase 1 and 2; eNos, endothelial nitric oxide synthase; nNos, neuronal nitric oxide synthase; Sod1, Sod2, and Sod3, superoxide dismutase 1, 2, and 3; Cald1, caldesmon 1; smMHC, smooth muscle myosin heavy chain; Mlck, myosin light-chain kinase; Smtn, smoothelin; Tpm1, tropomyosin 1; Cav1.2 and Cav1.3, voltage-dependent Ca²⁺ channel 1.2 and 1.3; BK, large-conductance Ca²⁺-activated K⁺ channel; Adrb2 and Adrb3, β₂- and β₃-adrenoceptor; Chrm2 and Chrm3, muscarinic receptor 2 and 3; ChAT, choline acetyltransferase; P2X1, purinergic receptor P2X 1; Myl9, myosin light chain 9; P-Myl9, Ser¹⁹-phosphorylated Myl9; SMA, smooth muscle actin; Tagl, transgelin.