Fig. 5.

Immunohistochemical analysis of young (3–4 mo old) and old (22 mo old) wild-type (WT) and lysyl oxidase-like 2 (LOXL2) littermate mouse aorta. All images shown are representative of images from 6 independent mouse aortas (×10 magnification) as whole rings. Insets: ×40 for each specimen. A–V: hematoxylin and eosin (H&E) stain (A), Masson trichrome stain (B), and Movat pentachrome stain (C). Scale bars = 50 μm. D: aging-related changes in nuclear content and wall thickness of aortas of WT and LOXL2^+/− mice. *P < 0.05, **P < 0.01, and ***P < 0.001.