Fig. 4.

Contribution of [U-¹³C]glycerol to de novo triglyceride (TG) synthesis according to the different timing of tracer ingestion. Excess ¹³C enrichment in TG glycerol moieties was negligible at 240 min when the glycerol drink was given at t = 210 min. However, the enrichment was detectable and stable from t = 240–390 min when given at t = 180 min (A). Contribution from direct vs. indirect pathway to de novo TG synthesis when the glycerol tracer is given at 180 min (B). Contribution from direct vs. indirect pathway to de novo TG synthesis when the glycerol tracer is given at 210 min (C). Data are presented as means (SD). ^aP < 0.05 compared with t = 240 min within the same glycerol drink timing condition; ^bP < 0.05 compared with t = 300 within the same glycerol drink timing condition. *P < 0.05 compared with the same time point between glycerol drink t = 180 vs. t = 210 min.