Figure 1.

Mice lacking the Bbs1 gene in POMC neurons develop obesity. Weekly body weights of female (A) and male (B) mice (n = 12–16/group: 12 males and 15 females for control [CTL] mice, 16 males and 16 females for POMC^cre/Bbs1^fl/fl mice). C–E: Fat mass and lean mass, measured by MRI (n = 7/group: three males and four females in each group). F and G: Cumulative daily food intake (n = 6–13/group: 6 males and 12 females for control and 6 males and 13 females for POMC^cre/Bbs1^fl/fl mice). H: Resting metabolic rate (RMR) (n = 4–6/group: six males and four females in each group), analyzed by univariate regression modeling (model, P < 0.001; body mass, P < 0.001; genotype, P = 0.912; sex, P = 0.501; interaction P = 0.453; body mass evaluated at 34.263 g; R² = 0.772). I and J: Plasma insulin levels. K and L: GTT (n = 10–12/group: 5 males and 5 females for controls and 6 males and 6 females for POMC^cre/Bbs1^fl/fl mice). M: Hypothalamic mRNA levels of metabolic neuropeptides (n = 6/group: three males and three females in each group). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. control group.