Figure 2.

Bbs1 gene deletion in AgRP neurons causes obesity. Weekly body weights of female (A) and male (B) mice (n = 10–23/group: 19 males and 23 females for controls [CTL] and 15 males and 10 females for AgRP^cre/Bbs1^fl/fl mice). C and D: Fat and lean masses. E and F: Plasma insulin levels. G and H: Hypothalamic mRNA levels of metabolic neuropeptides (n = 6/group). I: Selective loss of ciliary localization of NPY₂R in td-Tomato⁺ neurons of the hypothalamic arcuate nucleus of AgRP^cre/Bbs1^fl/fl mice. *P < 0.05 vs. control group. Scale bars, 10 µm.