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. 2019 Jun 20;20(8):3104–3114. doi: 10.1021/acs.biomac.9b00681

Figure 1.

Figure 1

Polymer synthesis and ice activity. (a) Synthesis of poly(ampholyte)s used. (b) IRI assay of P1–P3. MGS compared to a PBS control after 30 min of annealing at −8 °C. Error bars represent ±SEM of three repeats. (c) Enthalpy of crystallization (proportional to ice volume) for cryopreservation solutions containing P2 (NP = no polymer) via DSC at a cooling rate of 1 °C·min–1. (N = 24, P = 0.0000000004, *P < 0.001 from NP w/0% DMSO, **P < 0.001 from NP w/10% DMSO). Error bars represent ±SEM of three repeats.