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. 2018 Jan 29;99(2):384–394. doi: 10.1093/biolre/ioy020

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© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

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Figure 8. — Immunofluorescence using GSK3α and GSK3β monoclonal antibody shows expression of GSK3α (8A) and GSK3β (8B) in wild-type sperm. (A and B) (I) Bright field, (II) Hoechst/nucleus, (III) cyanine 3/GSK3α or GSK3β, (IV) merged, (VI) zoomed and merged with cyanine 3 to show the sperm head and Hoechst and (VII) zoomed and bright field merged with cyanine 3 and Hoechst. GSK3α is present in the tail and acrosomal region in the head. However, GSK3β is present in postacrosomal and equatorial region in head.