Table 1.
Kinetic parameters reported for NapA (A) or NapAB (AB) in various organisms using the methyl/benzyl viologen solution assay.
| Organism | pH | KM (μM) | kcat (s−1) | Vmax (μmoles nitrate min−1 mg−1) | kcat/KM (M−1 s−1) | Reference |
|---|---|---|---|---|---|---|
| Campylobacter jejuni (A) | 7.00 | 3.40 ± 0.44 | 5.91 ± 0.18 | 3.40 ± 0.10 | 1.74 × 106 | This work |
| Rhodobacter sphaeroides (A) | 7.00 | 120.00 | 70.20a | 39.00 | 5.85 × 105 | Sabaty et al. (2001) |
| Paracoccus pantotrophus (AB) | 7.00 | 112.00 | 58.00 | 5.18 × 105 | Gates, Richardson and Butt (2008) | |
| Paracoccus pantotrophus (AB) | 7.20 | 1300.00 | 240.00 | 1.85 × 105 | Butler et al. (1999) | |
| Magnetospirillum magnetotacticum (AB) | 7.00 | 3.20 | 2.50 | 7.81 × 105 | Taoka et al. (2003) | |
| Aliivibrio fischeri (AB) | 7.50 | 65.00 | 10.00a | 1.50 | 1.54 × 105 | Sadana and McElroy (1957) |
aValues calculated from kinetic parameters using the reported protein concentrations.