Figure 1.

Timeline for social behavior testing. Circles represent the round open field arena at each stage of behavioral testing, with wire mesh cups containing an ovariectomized stimulus mouse or no stimulus mouse (unmarked black circle). For each social behavior test, stimulus mice were added, and investigatory behavior was subsequently scored for 10 min. The mice were returned to their home cage for 40 min during the “home cage” period. SIT, social interaction test; SNT, social novelty test; SRT, social recognition test.