Table 2.

List of primers used for the construction of tethered eCG mutants

	Primer name	Location	Primer Sequence
1	eCGβ/α β-subnuit	-	5′- TGAATTCACCATGGAGACGGTCCAG -3′ Eco R I site
2	myc eCGβ/α reverse	-	5′- TCCATCAGGAAAAGAAGTCTTTATTGG -3′
3	myc eCGβ/α forward	-	5′- ATAAAGACTTCTTTTCCTTGATGGAGAG -3′
4	eCGβ/αΔ56 reverse	α 56	5′- TGAGGTGATCTGCTTTGGGACCAACAT -3′
5	eCGβ/αΔ56 forward	α 56	5′- ATGTTGGTCCCAAAGCAGATCACCTCA -3′
6	eCGβ/αΔ82 reverse	α 82	5′- GGCCTCCTTCTCAGCGGCCAGAGTGGCGTTGAT -3′
7	eCGβ/αΔ82 forward	α 82	5′- ATCAACGCCACTCTGGCCGCTGAGAAGGAGGCC -3′
8	eCGβΔ13/α reverse	β 13	5′- AGCAGCCAGAGTGGCGTTGATGGGCCGGCACAG -3′
9	eCGβΔ13/α forward	β 13	5′- CTGTGCCGGCCCATCAACGCCACTCTGGCTGCT -3′
10	eCGβ-D/α reverse	β : 121-149	5′- TCCATCAGGAAAGGCCTGGGGGGCACAGGC -3′
11	eCGβ-D/α forward	β : 121-149	5´- GCCCCCCAGGCCTTTCCTCATGGAGAGTTT -3´
12	eCGβ/α α-subunit	-	5′- CCGTCGACTTTAAATCTTGTGGTGATAGCA -3′ Sal I site