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. 2018 Mar 19;159(5):1972–1981. doi: 10.1210/en.2018-00081

Figure 2.

Figure 2.

The apparent competitive transcriptional activity of various oxysterols on human ERα with E2 in an estrogen response element (ERE) luciferase reporter inhibition screen in Ishikawa cells. (A) Antagonist activity of 100 nM, 1 μM, and 10 μM 27-OHC, 4β-OHC, and 7β-OHC on 100 pM E2 stimulation of ERE-driven luciferase expression. Error bars indicate standard error of the mean. *P < 0.05 compared with 100 pM E2-only control. (B) Antagonist activity of 100 nM, 1 μM, and 10 μM 7-ketocholesterol (7K-CHO), and 7α-OHC on 100 pM E2 stimulation of ERE-driven luciferase expression. (C) Antagonist activity of 10 nM, and 10 μM, 24(S)-OHC, and Faslodex (ICI-182-780) on 300 pM E2 stimulation of ERE-driven luciferase expression. Error bars indicate standard error of the mean. *P < 0.05 compared with 300 pM E2-only control.