Figure 5.

Coexpression of GDIα and SAD-A relieves the inhibitory effects of GDIα on GSIS. (a) Isolated mouse islets were infected or coinfected with recombinant adenoviruses expressing the indicated proteins (from left to right: vector, Flag-SAD-A and Myc-GDIα, kinase-dead SAD-A mutant K48M and Myc-GDIα, constitutively active SAD-A mutant T443A and Myc-GDIα, and Myc-GDIα) and were analyzed for GSIS by radioimmunoassay after 48 hours of infection. (b) Western blots analysis of islets after infected or coinfected with recombinant adenoviruses expressing the indicated proteins. **P < 0.01 and ***P < 0.001 when compared with vector control or indicated groups.