Table 1.
Key Resources
| Reagent or Resource | Source | Catalog |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| RhoGDI-GST protein (human recombinant) | Cytoskeleton | GDI01 |
| Human GDIα oligopeptides | Peptide 2.0 Inc. | Custom synthesis |
| Exendin-4 | Eli Lilly and Company | BYETTA® (exenatide) |
| X-tremeGENE™ HP DNA transfection reagent | Sigma-Aldrich | 6366546001 |
| ATP, [γ-32P]- 3000 Ci/mmol 10 mCi/mL lead, 250 µCi | PerkinElmer | NEG002A250UC |
| Coomassie stain | Bio-Rad | 1610786 |
| P81 phosphocellulose paper | Millipore | 20-134 |
| shRNA SAD-A | Franck Polleux | PMID: 17482548 |
| Collagenase XI | Sigma-Aldrich | C7657 |
| Histopaque®-1077 | Sigma-Aldrich | 10771 |
| Protease inhibitor cocktail tablets | Millipore | PICT |
| Protein G sepharose | GE Healthcare | 17061805 |
| Experimental models: organisms/stains | ||
| 8-week-old male C57BL/6J mice | The Jackson Laboratory | Stock No.: 000664 |
| Critical commercial assays | ||
| Pierce BCA protein assay kit | Thermo Fisher | 23225 |
| Pierce™ ECL | Thermo Fisher | 32106 |
| Pierce™ ECL Plus | Thermo Fisher | 32132 |
| Insulin radioimmunoassay kit | Millipore | RI-13K |
| Other | ||
| IKA® RW 20 digital | IKA® Works, Inc. | RW 20 digital |
Abbreviations: BCA, bicinchoninic acid assay; ECL, enhanced chemiluminescence; PMID, PubMed Identifier; shRNA, short hairpin RNA.