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. 2019 Aug 9;11:7577–7585. doi: 10.2147/CMAR.S210825

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© 2019 Xu et al.

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Evaluation of the effects of irradiation on the subcellular location of YAP1 protein and the interactions of YAP1 protein with nuclear transcription factors. U251 cells were exposed to 4 Gy irradiation, then the cells were collected after 24 hr and subjected to the following assays. (A, B) Western blotting assay was used to detect the expression of YAP1 in nuclear and cytoplasm. (C) Immunofluorescence technology was used to assess the effect of 4 Gy irradiation on the subcellular location of YAP1 protein in U251 cells. (D) Co-IP assay was used to detect the interaction between YAP1 protein and TEAD, CREB, P73 and RUNX2 proteins, IgG was used as negative controls. (E.) Western blotting analysis of the expression of p-YAP1 after cells was treated with 4 Gy irradiation (n=3, *P<0.05).

Abbreviations: CREB, cAMP responsive element binding protein 1; RUNX2, runt-related transcription factor 2; TEAD, TEA/ATTS domain; YAP, Yes-associated protein; Co-IP, co-immunoprecipitation.