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. 2019 Aug 9;11:7577–7585. doi: 10.2147/CMAR.S210825

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© 2019 Xu et al.

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The ubiquitination of YAP1 protein was regulated by RCHY1. U251 cells were treated with 4 Gy irradiation, then the following assays were carried out after 24 hrs of irradiation exposure. (A, B) The expression of TrCP, CDC4, RCHY1, MDM2, SKP2, UBE3A and SMURF1 was detected by Western blotting assay. (C) Co-IP assay was used to detect the effects of RCHY1 overexpression on the ubiquitination of YAP1 protein (n=3, *P<0.05).

Abbreviation: YAP, Yes-associated protein.