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. 2019 Aug 9;11:7577–7585. doi: 10.2147/CMAR.S210825

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© 2019 Xu et al.

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Downregulation of RCHY1 weakened the effect of irradiation on YAP1 downregulation and cell apoptosis induction. (A) The knockdown efficiency of si-RCHY1 was assessed by Western blotting assay after 48 hrs of cell transfection. Next, cells were transfected with si-RCHY1, following by irradiation exposure, then the following experiments were carried out. (B) The expression of YAP1 protein was assessed by Western blotting assay after 24 hrs of irradiation exposure. (C) Cell viability was detected by CCK-8 assay after 24 hrs of irradiation exposure. (D) Cell apoptosis was tested by flow cytometry assay after 24 hrs of irradiation exposure (n=3, *P<0.05).

Abbreviations: YAP, Yes-associated protein; CCK-8, Cell counting kit-8.