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. 2019 Aug 13;18:135. doi: 10.1186/s12934-019-1186-x

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Fermentation profiles of the E. coli LZ08 harboring plasmids pQQ05 and pZQ06 and mcl-PHA production. a Monomer composition and molar content of accumulated PHA. The percentage ratios of PHA produced are also shown. b Strain growth and glucose consumption were displayed in curve graph, while total content of PHA of the recombinant strain were determined at the end of fermentation and were indicated using histogram. c Acetate secretion of LZ05 (pQQ05, pZQ06) and LZ08 (pQQ05, pZQ06). Fermentations were conducted in shake flasks at 30 °C and 250 rpm with 30 g/L glucose and 2.0 g/L propionate. The experiments were performed in triplicate, and error bars indicate standard deviation (SD). Statistical analysis on the figure shows comparison of acetate secretion between LZ05 (pQQ05, pZQ06) and LZ08 (pQQ05, pZQ06). The *** indicate P < 0.001