Table 1.
Strains and plasmids used in this study
| Strains and plasmids | Relevant genotype/property | Source or references |
|---|---|---|
| Strains | ||
| E. coli DH5a | F−, endA1, hsdR17, (rk−, mk+), supE44, thi-1, λ−, recA1, gyrA96, ΔlacU169 (Φ80 lacZ ΔM15) | Laboratory stock |
| E. coli LS5218 | F+, fadR601, atoC512 (Const) | Laboratory stock |
| LZ05 | E. coli LS5218 ΔptsG::FRT ΔtesB::FRT ΔyciA::FRT | Zhuang et al. [23] |
| LZ08 | E. coli LS5218 ΔptsG::FRT ΔtesA::FRT ΔpflB::FRT ΔpoxB::FRT | This study |
| Plasmids | ||
| pBBR1MCS-2 | lacPOZ mobRP4, low-copy-no. cloning vector; KmR | Kovach et al. [37] |
| pQQ05 | pTrc99a derivative, yqeF and fadB from E. coli MG1655, phaJ1 Pa and phaC2 Pa from P. aeruginosa PAO1, ter from Treponema denticola | Zhuang et al. [23] |
| pZQ01 | pBBR1MCS2-prpP; pBBR1MCS-2 derivative, prpP from R. eutropha H16 | This study |
| pZQ02 | pBBR1MCS2-acs; pBBR1MCS-2 derivative, acs from E. coli MG1655 | This study |
| pZQ03 | pBBR1MCS2-prpE; pBBR1MCS2-derivative, prpE from R. eutropha H16 | This study |
| pZQ04 | pBBR1MCS2-pct; pBBR1MCS-2 derivative, pct from R. eutropha H16 | This study |
| pZQ05 | pBBR1MCS2-prpP-acs; pBBR1MCS-2 derivative, prpP from R. eutropha H16 and acs from E. coli MG1655 | This study |
| pZQ06 | pBBR1MCS2-prpP-prpE; pBBR1MCS2-derivative, prpP and prpE from R. eutropha H16 | This study |
| pZQ07 | pBBR1MCS2-prpP-pct; pBBR1MCS2-derivative, prpP and pct from R. eutropha H16 | This study |