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. 2019 Aug 13;19:350. doi: 10.1186/s12870-019-1945-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Loss of GhYGL1d expression affects chloroplast development. a-h Chlorophyll fluorescence and morphology of the protoplasts isolated from the CLCrVA (a, e), GhYGL1d-RNAi-1(b, f), GhYGL1d-RNAi-2 (c, g) and GhYGL1d-RNAi-3 (d, h) plant leaves. (i-l) Transmission electron micrographs of plastid ultrastructures in the CLCrVA (I), GhYGL1d-RNAi-1(J), GhYGL1d-RNAi-2 (K) and GhYGL1d-RNAi-3 (L) plants. Plastids were from leaves of six-week-old plants. Three biological replicates were performed, and similar results were obtained. SG, starch grain. Thy, thylakoid. Gr, granum. Bars = 1 μm.