Fig. 5.

sFGL2 downregulates inflammatory marker expression on DCs. Bone-marrow cells were isolated from BALB/c mice and stimulated with 10 ng/mL IL-4 and 10 ng/mL GM-CSF for 6 days in order to generate immature BMDCs, which were then a incubated with or without 4 μg/mL of recombinant murine FGL2 for 16 h, followed by 0.5 μg/mL LPS stimulation for 12 h. b MHCII, c CD40, d CD80, e CD86, f CD83, and g CD31 presented on the DC surface were measured by flow cytometry. Data represent the mean ± SEM. *P < 0.05, **P < 0.01 (Mann–Whitney U test) and compared with the control